EGFR antibody LS-B2199 is an unconjugated sheep polyclonal antibody to EGFR from human, mouse, rat and other species. Validated for ELISA, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Human, Mouse, Rat, Chicken
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin (5 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
EGFR antibody was raised against recombinant 20aa residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region.
Recognizes human Epidermal Growth Factor Receptor.
Immunohistochemistry: LS-B2199 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2199 was determined to be 5 ug/ml.
0.15 M PBS, pH 7.4, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.