Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Protein G purified
EGFR antibody was raised against purified EGFR from A431 cells.
Recognizes the 170kD (wild type) and 145kD (VIII variant) forms of human EGF receptor. The epitope maps to the extracellular domain of EGFR. Species cross-reactivity: chimpanzee and monkey. Does not react with mouse or rat.
Suitable for use in Immunoprecipitation, Blocking EGT/TGFa-Induced Activation of EGFR and also Inhibits binding of E3375-03. Blocking EGF/TGFa-induced activation of EGFR: Use 0.5 ug/ml, cells induced by 30 ng/ml EGF. Immunoprecipitation (native only): 2 ug/mg protein lysate. Use Protein G. Positive control: A431 cells.
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues.