(tested or 100% immunogen sequence identity)
Protein G purified
EGFR antibody was raised against purified EGFR from A431 cells.
Recognizes the 170kD (wild type) and 145kD (VIII variant) forms of human EGF receptor. The epitope maps to the extracellular domain of EGFR. Species cross-reactivity: chimpanzee and monkey. Does not react with mouse or rat.
Suitable for use in Immunoprecipitation, Blocking EGT/TGFa-Induced Activation of EGFR and also Inhibits binding of E3375-03. Blocking EGF/TGFa-induced activation of EGFR: Use 0.5 ug/ml, cells induced by 30 ng/ml EGF. Immunoprecipitation (native only): 2 ug/mg protein lysate. Use Protein G. Positive control: A431 cells.
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.