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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EGFR Antibody (Extracellular Domain, clone 225) LS‑C6560
EGFR antibody LS-C6560 is an unconjugated mouse monoclonal antibody to EGFR from human and monkey. Validated for IP.
Antibody
Catalog
Size
Price
LS-C6560-500
500 µl (0.2 mg/ml)
$620

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Product Description

EGFR antibody LS-C6560 is an unconjugated mouse monoclonal antibody to EGFR from human and monkey. Validated for IP.
About EGFR
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. P00533 NM_005228 NP_005219.2

EGFR Antibody, EGFf receptor Antibody, ERBB Antibody, ERBB1 Antibody, MENA Antibody, PIG61 Antibody, Proto-oncogene c-ErbB-1 Antibody, V-erb-b homolog Antibody, EGF-R Antibody, HER1 Antibody


Specifications

Target
Human EGFR
Host
Mouse
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [225]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Immunoprecipitation
Immunogen
EGFR antibody was raised against purified EGFR from A431 cells.
Epitope
Extracellular Domain
Specificity
Recognizes the 170kD (wild type) and 145kD (VIII variant) forms of human EGF receptor. The epitope maps to the extracellular domain of EGFR. Species cross-reactivity: chimpanzee and monkey. Does not react with mouse or rat.
Usage
Suitable for use in Immunoprecipitation, Blocking EGT/TGFa-Induced Activation of EGFR and also Inhibits binding of E3375-03. Blocking EGF/TGFa-induced activation of EGFR: Use 0.5 ug/ml, cells induced by 30 ng/ml EGF. Immunoprecipitation (native only): 2 ug/mg protein lysate. Use Protein G. Positive control: A431 cells.
Presentation
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/21/2018

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