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Anti-EGFR Antibody (C-Terminus) IHC-plus™ LS-A9400

Note: This antibody replaces LS-C65434, LS-C65407, LS-C65406

Ordering

Wt. Vol. Conc. Price
50 µg 62.5 µl 0.8 mg/ml Unavailable
Same day shipping In Stock - Same day shipping

Most Popular EGFR Antibodies

Anti-EGFR Antibody (clone H11) LS-C26773
Mouse Monoclonal [clone H11] (IgG1)
Human
IHC - Paraffin, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-EGFR Antibody (Internal) IHC-plus™ LS-B2199
Sheep Polyclonal (IgG) to Human EGFR
Human, Mouse, Rat, Chicken
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EGFR Antibody (clone 225, Azide-free) LS-C88001
Mouse Monoclonal [clone 225] (IgG1) to Human EGFR
Human, Monkey
Immunoprecipitation, Blocking, Inhibition
Unconjugated, Azide-free

100% Guaranteed 100% Guaranteed In Stock In Stock
Rabbit Polyclonal to Human EGFR
Human
IHC - Paraffin
Unconjugated

Details

Human EGFR
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey, Rat (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

IHC - Paraffin (2.5 µg/ml)

Specificity and Use

EGFR antibody was raised against synthetic 16 amino acid peptide from C-terminus of human EGFR. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Marmoset, Rat (94%); Mouse (88%); Bovine, Dog, Hamster, Elephant, Horse (81%).
LS-E29635 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A9400. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
Human EGFR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A9400 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A9400 was determined to be 2.5 ug/ml.

Packaging

PBS, less than 0.1% sodium azide.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
For research use only.

About EGFR

P00533 NM_005228 NP_005219.2

EGFR Antibody, EGFf receptor Antibody, ERBB Antibody, ERBB1 Antibody, MENA Antibody, PIG61 Antibody, Proto-oncogene c-ErbB-1 Antibody, V-erb-b homolog Antibody, EGF-R Antibody, HER1 Antibody

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues.

Immunohistochemistry

Immunohistochemistry
Anti-EGFR antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Immunohistochemistry
Anti-EGFR antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: 
Date Requested: 3/29/2017

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