Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Mouse Monoclonal (IgG1,k) to Human EGFR
Western blot, Immunoprecipitation, ELISA
Human, Mouse (tested or 100% immunogen sequence identity)
Western blot (1 µg/ml)
ELISA (0.05 µg/ml)
Specificity and Use
EGFR antibody was raised against synthetic peptide corresponding to aa 1165-1185 of EGFR (epidermal growth factor receptor).
Recognizes the C-terminus (aa 1165-1186) of human, mouse and rat EGFR.
Suitable for use in ELISA, Immunoprecipitation and Western Blot. ELISA: 0.05 ug/ml. Western Blot: 1 ug/ml for AP (BCAP, NBI) (DTT), 0.1 ug/ml for (ECL). Immunoprecipitation: 1-10 ug/1E6 vanadate treated A431 cells. Positive control: H2030-03H, HepG2 Cell Lysate, Untreated.
PBS, pH 7.4, 0.09% sodium azide, PEG, sucrose.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues.