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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
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Seattle WA 98121

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EGFR Antibody (aa1165‑1185) LS‑C6635
EGFR antibody LS-C6635 is an unconjugated mouse monoclonal antibody to EGFR from human and mouse. Validated for ELISA, IP and WB.
Antibody
Catalog
Size
Price
LS-C6635-100
100 µg
$710

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Product Description

EGFR antibody LS-C6635 is an unconjugated mouse monoclonal antibody to EGFR from human and mouse. Validated for ELISA, IP and WB.

Specifications

Target
Human EGFR
Synonyms
EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • Immunoprecipitation
  • ELISA (0.05 µg/ml)
Immunogen
EGFR antibody was raised against synthetic peptide corresponding to aa 1165-1185 of EGFR (epidermal growth factor receptor).
Epitope
aa1165-1185
Specificity
Recognizes the C-terminus (aa 1165-1186) of human, mouse and rat EGFR.
Usage
Suitable for use in ELISA, Immunoprecipitation and Western Blot. ELISA: 0.05 ug/ml. Western Blot: 1 ug/ml for AP (BCAP, NBI) (DTT), 0.1 ug/ml for (ECL). Immunoprecipitation: 1-10 ug/1E6 vanadate treated A431 cells. Positive control: H2030-03H, HepG2 Cell Lysate, Untreated.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, Sucrose, PEG
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About EGFR
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. P00533 NM_005228 NP_005219.2


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Requested From: United States
Date Requested: 10/15/2018

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