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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EGF Antibody (aa647‑696) LS‑C107618
EGF antibody LS-C107618 is an unconjugated rabbit polyclonal antibody to EGF from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C107618-50
50 µg
Unavailable

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Product Description

EGF antibody LS-C107618 is an unconjugated rabbit polyclonal antibody to EGF from human, mouse, rat and other species. Validated for WB.
About EGF
EGF is a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. P01133 NM_001963 NP_001954.2

EGF Antibody, Beta-urogastrone Antibody, HOMG4 Antibody, URG Antibody, Epidermal growth factor Antibody, Pro-epidermal growth factor Antibody


Specifications

Target
Human EGF
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Horse, Pig, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
EGF antibody was raised against synthetic peptide located between aa647-696 of human EGF (P01133, NP_001954). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%); Monkey, Marmoset, Panda, Bat, Horse, Pig, Turkey, Zebra finch, Chicken (92%); Xenopus (90%); Rat, Dog, Cat (85%); Lizard (84%); Mouse (78%).
Blocking Peptide
LS-E15273 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C107618. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa647-696
Specificity
Human EGF
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

EGF antibody LS-C107618 Western blot of COLO205 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF antibody LS-C107618 Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EGF antibody LS-C107618 Western blot of COLO205 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF antibody LS-C107618 Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EGF antibody LS-C107618 Western blot of COLO205 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF antibody LS-C107618 Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EGF antibody LS-C107618 Western blot of COLO205 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF antibody LS-C107618 Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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