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Anti-EGF Antibody (aa647-696) LS-C107618

Ordering

Wt. Vol. Conc. Price
50 µg - - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EGF Antibodies

Anti-EGF Antibody LS-C104712
Rabbit Polyclonal to Rat EGF
Rat
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-EGF Antibody (clone 9D7F11) IHC-plus™ LS-B4124
Mouse Monoclonal [clone 9D7F11] (IgG1) to Human EGF
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EGF Antibody (aa106-119) IHC-plus™ LS-B11905
Goat Polyclonal to Human EGF
Human, Monkey, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human EGF
Human, Mouse, Rat, Dog
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human EGF
Rabbit
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Monkey, Bat, Horse, Pig, Chicken, Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

  • IHC - Paraffin

Specificity and Use

EGF antibody was raised against synthetic peptide located between aa647-696 of human EGF (P01133, NP_001954). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%); Monkey, Marmoset, Panda, Bat, Horse, Pig, Turkey, Zebra finch, Chicken (92%); Xenopus (90%); Rat, Dog, Cat (85%); Lizard (84%); Mouse (78%).
LS-E15273 - Lyophilized - 100 µg - $145.00
aa647-696
Human EGF
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About EGF

P01133 NM_001963 NP_001954.2

EGF Antibody, Beta-urogastrone Antibody, HOMG4 Antibody, URG Antibody, Epidermal growth factor Antibody, Pro-epidermal growth factor Antibody

EGF is a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor.

Western blot

Western blot
EGF antibody LS-C107618 Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/10/2016

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