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Catalog Number Size Price
LS-C104654-50 50 µg $356 
LS-C104654-100 100 µg $396 
EGF Antibody - Western Blot (reducing) of EGF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF Antibody - Western Blot (non-reducing) of EGF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF Antibody - Sandwich ELISA of EGF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF Antibody - Sandwich ELISA of EGF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF Antibody - Western Blot (reducing) of EGF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF Antibody - Western Blot (non-reducing) of EGF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF Antibody - Sandwich ELISA of EGF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EGF Antibody - Sandwich ELISA of EGF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Rabbit anti‑Human EGF Antibody (WB) LS‑C104654

Polyclonal Rabbit anti‑Human EGF Antibody (WB) LS‑C104654

Note: This antibody replaces LS-C54001, LS-C23132
Antibody:
EGF Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$356
LS-C104654-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
EGF Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
EGF antibody LS-C104654 is an unconjugated rabbit polyclonal antibody to human EGF. Validated for ELISA and WB.
Target
Human EGF
Synonyms
EGF | Beta-urogastrone | HOMG4 | URG | Epidermal growth factor | Pro-epidermal growth factor
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human EGF.
Specificity
Human EGF
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Usage
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 ug/ml-0.25 ug/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hEGF (25 pg/ml), a concentration of < 0.1 ug/ml of this antibody is required. ELISA: To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (LS-C104809) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EGF
P01133 NM_001963 NP_001954.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/10/2024