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Anti-ECT2 Antibody (phospho-Thr790) LS-C19111


Wt. Vol. Conc. Price
100 µg - 1.31 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular ECT2 Antibodies

Anti-ECT2 Antibody (aa166-348, FITC) LS-C303856
Rabbit Polyclonal (IgG) to Human ECT2
Western blot, ELISA
FITC Conjugated
Western blot Image
Anti-ECT2 Antibody (aa453-647, FITC) LS-C304650
Rabbit Polyclonal (IgG) to Human ECT2
Western blot, ELISA
FITC Conjugated
Western blot Image
Anti-ECT2 Antibody (aa175-438, clone 2D9) LS-C340261
Mouse Monoclonal [clone 2D9] (IgG1) to Human ECT2
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ECT2
Human, Mouse, Rat, Dog, Chicken, Zebrafish
Western blot, Immunoprecipitation, ELISA


Human ECT2
Human, Mouse, Rat, Dog, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Western blot (1:1000 - 1:4000)
  • Immunoprecipitation
  • ELISA (1:10000 - 1:40000)

Specificity and Use

ECT2 antibody was raised against synthetic peptide from human ECT2.
Amino acids 785-795 of human ECT2 protein.
This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 101 kD in size by western blotting in the appropriate cell lysate or extract. Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is believed to be phospho specific for ECT2 phosphorylated at the pT790 residue. Experiments to generate more conclusive evidence are currently under preparation.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About ECT2

Q9H8V3 NM_018098 NP_060568.3

ECT2 Antibody, ARHGEF31 Antibody, Protein ECT2 Antibody

Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis.

Western blot

Western blot
Anti-ECT2 pT790 Antibody - Western Blot. Western blot of affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and non-phosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1000. Personal Communication, Toru Miki, CCR-NCI, Bethesda, MD.

Requested From: United States
Date Requested: 10/20/2016

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