LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
E2F1 Antibody (clone 8G9) LS‑C169098
E2F1 antibody LS-C169098 is an unconjugated mouse monoclonal antibody to human E2F1. Validated for ELISA, Flow and IHC.
Catalog
Size
Price
LS-C169098-100
100 µg (1 mg/ml)
Unavailable

Popular E2F1 Products

Antibody
Species: Human, Xenopus
Applications: Western blot, Immunoprecipitation
Anti-E2F1 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B52 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of the cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Anti-E2F1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

E2F1 antibody LS-C169098 is an unconjugated mouse monoclonal antibody to human E2F1. Validated for ELISA, Flow and IHC.
About E2F1
E2F-1 (also known as transcription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Q01094 NM_005225 NP_005216.1

E2F1 Antibody, E2F transcription factor 1 Antibody, PRB-binding protein E2F-1 Antibody, RBAP-1 Antibody, RBBP3 Antibody, RBBP-3 Antibody, Transcription factor E2F1 Antibody, RBAP1 Antibody, E2F-1 Antibody, PBR3 Antibody


Specifications

Target
Human E2F1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [8G9]
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
E2F1 antibody was raised against purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.\ \
Specificity
Human, Mouse
Presentation
Purified antibody in PBS containing 0.05% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

IHC of paraffin-embedded esophageal cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophageal cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded rectum cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using E2F1 mouse monoclonal antibody against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
Western blot using E2F1 mouse monoclonal antibody against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.

Western blot

Western blot using E2F1 monoclonal antibody against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)
Western blot using E2F1 monoclonal antibody against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded esophageal cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophageal cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded rectum cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using E2F1 mouse monoclonal antibody against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
Western blot using E2F1 mouse monoclonal antibody against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.

Western blot

Western blot using E2F1 monoclonal antibody against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)
Western blot using E2F1 monoclonal antibody against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded esophageal cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophageal cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded rectum cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using E2F1 mouse monoclonal antibody against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
Western blot using E2F1 mouse monoclonal antibody against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.

Western blot

Western blot using E2F1 monoclonal antibody against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)
Western blot using E2F1 monoclonal antibody against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded esophageal cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophageal cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded rectum cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using E2F1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using E2F1 mouse monoclonal antibody against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
Western blot using E2F1 mouse monoclonal antibody against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.

Western blot

Western blot using E2F1 monoclonal antibody against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)
Western blot using E2F1 monoclonal antibody against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);


Requested From: 
Date Requested: 6/22/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number