Target
Human DUOX
Synonyms
DUOX1 | DUOX | Flavoprotein NADPH oxidase | Long NOX 1 | NOXEF1 | Large NOX 1 | Thyroid oxidase 1 | NADPH thyroid oxidase 1 | THOX1 | Dual oxidase 1 | LNOX1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence CSRSDIETELTPQR, from the internal region of the protein sequence according to NP_059130.2.
Specificity
Human DUOX. Reported variants represent identical protein: NP_787954.1, NP_059130.2. This antibody is not expected to cross-react with DUOX2.
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 170kD band observed in Human Testis lysates (calculated MW of 177kD according to NP_059130.2). Recommended concentration: 0.1-0.3 ug/ml. An additional band of unknown identity was also consistently observed at 140kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee