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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DPPA5 Antibody LS‑C36163
DPPA5 antibody LS-C36163 is an unconjugated goat polyclonal antibody to mouse DPPA5. Validated for ELISA, ICC and WB.
Antibody
Catalog
Size
Price
LS-C36163-100
100 µg
$725

Popular DPPA5 Products

DPPA5 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The DPPA5 antibody detected the DPPA5 protein (arrow).
Species: Human
Applications: Western blot, Flow Cytometry
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA

Product Description

DPPA5 antibody LS-C36163 is an unconjugated goat polyclonal antibody to mouse DPPA5. Validated for ELISA, ICC and WB.
About DPPA5
Involved in the maintenance of embryonic stem (ES) cell pluripotency. Dispensable for self-renewal of pluripotent ES cells and establishment of germ cells. Associates with specific target mRNAs. A6NC42 NM_001025290 NP_001020461.1

DPPA5 Antibody, ESG-1 Antibody, HDPPA5 Antibody, ESG1 Antibody


Specifications

Target
Mouse DPPA5
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot
  • ELISA
Immunogen
DPPA5 antibody was raised against e. coli-derived rmDPPA5.
Specificity
Selected for its ability to recognize mouse DPPA5 in the applications listed.
Usage
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 25% cross-reactivity with rhDPPA5 and approximately 10% cross-reactivity with rmDPPA4. This antibody has not been optimized for Western blot detection of DPPA5 in cell lysates. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect mouse DPPA5 in mouse embryonic stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 0.3 ng/well.
Presentation
Lyophilized
Reconstitution
100 µl deionized water
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/20/2018

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