(tested or 100% immunogen sequence identity)
DPPA5 antibody was raised against e. coli-derived rmDPPA5.
Selected for its ability to recognize mouse DPPA5 in the applications listed.
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 25% cross-reactivity with rhDPPA5 and approximately 10% cross-reactivity with rmDPPA4. This antibody has not been optimized for Western blot detection of DPPA5 in cell lysates. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect mouse DPPA5 in mouse embryonic stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 0.3 ng/well.