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Anti-DLL4 Antibody (Internal) LS-C19035

Note: This antibody replaces LS-C64649

Ordering

Wt. Vol. Conc. Price
100 µg - 0.93 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular DLL4 Antibodies

Anti-DLL4 Antibody (Internal) LS-C19035
Rabbit Polyclonal to Human DLL4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-DLL4 Antibody (clone 4A11) LS-C169100
Mouse Monoclonal [clone 4A11] (IgG1) to Human DLL4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal to Human DLL4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human DLL4
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin
  • Western blot (1:500 - 1:2500)
  • ELISA (1:5000 - 1:25000)

Specificity and Use

DLL4 antibody was raised against synthetic peptide from human DLL4.
Internal
An internal region of Human DELTA-4.
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 74 kD in size corresponding to DELTA-4 by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (2)

1
Anthrax toxins cooperatively inhibit endocytic recycling by the Rab11/Sec15 exocyst. Guichard A, McGillivray SM, Cruz-Moreno B, van Sorge NM, Nizet V, Bier E. Nature. 2010 467:854-8. (ICC; Human) [PubMed:20944747]
2
Cholera toxin disrupts barrier function by inhibiting exocyst-mediated trafficking of host proteins to intestinal cell junctions. Guichard A, Cruz-Moreno B, Cruz-Moreno BC, Aguilar B, van Sorge NM, Kuang J, Kurkciyan AA, Wang Z, Hang S, Pineton de Chambrun GP, McCole DF, Watnick P, Nizet V, Bier E. Cell host & microbe. 2013 Sep;14:294-305. [Full Text Article] [PubMed:24034615] [PMC:PMC3786442]

About DLL4

Q9NR61 NM_019074 NP_061947.1

DLL4 Antibody, Delta-like 4 protein Antibody, Delta like 4 Antibody, Delta-like 4 (Drosophila) Antibody, Delta-like 4 homolog Antibody, Drosophila Delta homolog 4 Antibody, Notch ligand DLL4 Antibody, Delta 4 Antibody, Delta ligand 4 Antibody, Delta-like protein 4 Antibody, Delta4 Antibody, Hdelta2 Antibody, Notch ligand delta-2 Antibody

Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation is required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate.

Immunohistochemistry

Immunohistochemistry
Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Western blot

Western blot
Western Blot - Anti-Delta-4 Antibody. Western blot of Affinity Purified anti-Delta-4 antibody shows detection of a 74-kD band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Delta-4 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3 min exposure time.

Requested From: United States
Date Requested: 12/5/2016

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