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Anti-DBT / E2 Antibody (clone 1G2) LS-C173946

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $350
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DBT / E2 Antibodies

Anti-DBT / E2 Antibody (aa35-84) IHC-plus™ LS-B4677
Rabbit Polyclonal to Human DBT / E2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DBT / E2 Antibody (clone 1G2) LS-C173946
Mouse Monoclonal [clone 1G2] (IgG1) to Human DBT / E2
Human
Western blot, Flow Cytometry
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1G2] (IgG1) to Human DBT / E2
Human
Western blot, Flow Cytometry
Unconjugated

Details

Human DBT / E2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1G2]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

DBT / E2 antibody was raised against full length human recombinant protein of human DBT(NP_001909) produced in HEK293T cell.
Human DBT

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About DBT / E2

P11182 NM_001918 NP_001909.3

DBT Antibody, BCKAD E2 subunit Antibody, BCKAD-E2 Antibody, BCKADE2 Antibody, Dihydrolipoyl transacylase Antibody, E2 Antibody, E2B Antibody, BCATE2 Antibody

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DBT antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 12/4/2016

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