Target
Human DAXX
Synonyms
DAXX | BING2 | CENP-C binding protein | DAP6 | Death-associated protein 6 | EAP1 | Fas-binding protein | HDaxx | ETS1-associated protein 1
Reactivity
Human, Mouse, Rat, Hamster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
GST fusion protein corresponding to residues 472-740 of human Daxx.
Specificity
Recognizes Daxx, Mr ~110kD. An unknown band was also detected, Mr ~150kD. The identity of the Mr ~110kD band as Daxx was confirmed by an independent laboratory using a siRNA strategy. Species cross-reactivity. Human, mouse, rat and Chinese hamster.
Applications
- ICC (1:100)
- Western blot (1:5000)
- Immunoprecipitation
Usage
Suitable for use in ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry and Immunohistochemistry. Immunoprecipitation: 5 ul immunoprecipitates Daxx from 150 ug of total soluble protein. Western Blot: 1:5000-1:1000 detects Daxx in HeLa nuclear extract. Immunohistochemistry, Immunocytochemistry: 1:100 shows positive immunostaining for Daxx in human cells fixed with 3.7% formaldehyde.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee