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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DAXX Antibody (aa261‑274) IHC‑plus™ LS‑B363 Best Seller
Note: This antibody replaces LS-C18933, LS-C56879
DAXX antibody LS-B363 is an unconjugated rabbit polyclonal antibody to DAXX from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B363-50
50 µg (1 mg/ml)
Unavailable

Popular DAXX Products

Antibody
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot
Confocal immunofluorescence of HeLa cells using DAXX mouse monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Species: Human
Applications: Flow Cytometry, ELISA
Anti-DAXX antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6992 dilution 1:67.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunofluorescence of monoclonal antibody to DAXX on HeLa cell . [antibody concentration 10 ug/ml]
Species: Human
Applications: Immunofluorescence, Western blot, ELISA

Product Description

DAXX antibody LS-B363 is an unconjugated rabbit polyclonal antibody to DAXX from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About DAXX
Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Q9UER7 NM_001350 NP_001341.1

DAXX Antibody, BING2 Antibody, CENP-C binding protein Antibody, DAP6 Antibody, Death-associated protein 6 Antibody, EAP1 Antibody, Fas-binding protein Antibody, HDaxx Antibody, ETS1-associated protein 1 Antibody


Specifications

Target
Human DAXX
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:200 - 1:2000)
  • ELISA (1:7000 - 1:45000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
DAXX antibody was raised against synthetic peptide from human Daxx.
Epitope
aa261-274
Specificity
Amino acids 261-274 of human DAXX protein.
Usage
Immunohistochemistry: LS-B363 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B363 was determined to be 2.5 ug/ml.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-DAXX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B363 concentration 5 ug/ml.
Anti-DAXX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B363 concentration 5 ug/ml.

Western blot

Anti-DAXX Antibody - Western Blot. Western blot of affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.
Anti-DAXX Antibody - Western Blot. Western blot of affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.

Immunohistochemistry

Anti-DAXX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B363 concentration 5 ug/ml.
Anti-DAXX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B363 concentration 5 ug/ml.

Western blot

Anti-DAXX Antibody - Western Blot. Western blot of affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.
Anti-DAXX Antibody - Western Blot. Western blot of affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.

Immunohistochemistry

Anti-DAXX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B363 concentration 5 ug/ml.
Anti-DAXX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B363 concentration 5 ug/ml.

Western blot

Anti-DAXX Antibody - Western Blot. Western blot of affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.
Anti-DAXX Antibody - Western Blot. Western blot of affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.

Immunohistochemistry

Anti-DAXX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B363 concentration 5 ug/ml.
Anti-DAXX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B363 concentration 5 ug/ml.

Western blot

Anti-DAXX Antibody - Western Blot. Western blot of affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.
Anti-DAXX Antibody - Western Blot. Western blot of affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.


Requested From: 
Date Requested: 6/21/2018

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