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Anti-DAXX Antibody (aa261-274) IHC-plus™ LS-B363

Note: This antibody replaces LS-C18933, LS-C56879


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

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Rabbit Polyclonal to Human DAXX
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA


Human DAXX
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Immunoaffinity purified


IHC - Paraffin (2.5 µg/ml), Immunofluorescence, Western blot (1:200 - 1:2000), ELISA (1:7000 - 1:45000)

Specificity and Use

DAXX antibody was raised against synthetic peptide from human Daxx.
Amino acids 261-274 of human DAXX protein.
Immunohistochemistry: LS-B363 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B363 was determined to be 2.5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About DAXX

Q9UER7 NM_001350 NP_001341.1

DAXX Antibody, BING2 Antibody, CENP-C binding protein Antibody, DAP6 Antibody, Death-associated protein 6 Antibody, EAP1 Antibody, Fas-binding protein Antibody, HDaxx Antibody, ETS1-associated protein 1 Antibody

Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby.


Anti-DAXX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B363 concentration 5 ug/ml.

Western blot

Western blot
Anti-DAXX Antibody - Western Blot. Western blot of affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.

Requested From: United States
Date Requested: 11/28/2015

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