Human CYCS / Cytochrome c
Human, Mouse, Rat, Rabbit
(tested or 100% immunogen sequence identity)
- Western blot
- Flow Cytometry
Human cytochrome c.
Recognizes cytochrome c, originally termed Apaf-2, an evolutionarily conserved protein which acts as an essential mitochondrial electron carrier between respirable substrates and oxygen, during aerobic energy production. A second major function for cytochrome c is its role as an intermediate in apoptosis, both through its association with inositol-3-phosphate receptor (IP3 receptor) which acts as an ER calcium release channel, and its association with apoptotic protease activating factor 1 (Apaf-1), resulting in the formation of the apoptosome protein complex which then recruits and activates pro-caspase 9 (Apaf-3). Clone 2G8 is reported to block the role of cytochrome c as a co-factor in the activation of caspase 9. Removal of sodium azide is recommended prior to use in functional assays.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Method sheets are available on request.
0.09% sodium azide.
Short term: -20°C; Long term: -20°C.
For research use only.
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