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Anti-CXCR3 Antibody (clone 2AR1) LS-C6283


Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml Unavailable

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100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 2AR1] (IgG1) to Human CXCR3
IHC, Flow Cytometry, Functional Assay, Neutralization


Human CXCR3
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2AR1]
Protein G purified


  • IHC (10 µg/ml)
  • Flow Cytometry (5 - 10 µg/ml)
  • Functional Assay
  • Neutralization

Specificity and Use

CXCR3 antibody was raised against cXCR3 transfected NS0 cells.
Recognizes human CXCR3. Reacts specifically with CXCR3 transfectants and not the parental cell line using FACS analysis. Does not cross-react with CXCR1, CXCR2, CXCR4 or transfectants.
Suitable for use in Neutralization, Flow Cytometry and Immunohistochemistry. Neutralization (ND50): The exact concentration required to neutralize human cell surface CXCR3 activity is dependent on the ligand concentration as well as the number of CXCR3 receptors present on the cell surface. The Neutralization Dose50 (ND50) is defined as the concentration of antibody required to yield one half maximal inhibition of the cell surface CXCR3 mediated rhl-TAC response on a responsive cell line, at a specific rhl-TAC concentration. The ND50 for this lot was determined to be ~1-6 ug/ml in the presence of 7 ng/ml of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay. Flow Cytometry: 5-10 ug/ml; Add 10 ul to 1-2.5x10^5 cells in total reaction volume 200ul. Visualize binding by adding 10 ul of 25 ug/ml IgG (FITC) goat anti-mouse. Immunohistochemistry: 10 ug/ml detected CXCR3 in human tonsil. Cells were fixed in PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. Cells were washed with PBS, BSA.


Lyophilized from PBS, 5% trehalose.
200 µl 200ul sterile PBS
Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
For research use only.

Publications (1)

Tumor-associated lymphocytes as an independent predictor of response to neoadjuvant chemotherapy in breast cancer. Denkert C, Loibl S, Noske A, Roller M, Mller BM, Komor M, Budczies J, Darb-Esfahani S, Kronenwett R, Hanusch C, von Trne C, Weichert W, Engels K, Solbach C, Schrader I, Dietel M, von Minckwitz G. Journal of clinical oncology : official journal of the American Society of Clinical Oncology. 2010 28:105-13. (IHC-P; Human) [PubMed:19917869]

About CXCR3

P49682 NM_001504 NP_001495.1

CXCR3 Antibody, C-x-c chemokine receptor 3 Antibody, CD183 antigen Antibody, CKR-L2 Antibody, CXCR-3 Antibody, CMKAR3 Antibody, CD182 Antibody, CD183 Antibody, Cxcr3a Antibody, GPR9 Antibody, IP-10 receptor Antibody, IP10-R Antibody, Mig receptor Antibody, MigR Antibody, Mig-R Antibody, Chemokine (C-X-C) receptor 3 Antibody, CXC-R3 Antibody, Cxcr3b Antibody, G protein-coupled receptor 9 Antibody, IP10 receptor Antibody

CXCR3 is a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration.

Requested From: 
Date Requested: 4/27/2017

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