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Anti-CXCL5 Antibody LS-C8497

Ordering

Wt. Vol. Conc. Price
100 µg - 0.2 mg/ml $695
Inquire for larger quantities

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Goat Polyclonal to Human CXCL5
Human
IHC - Paraffin, Western blot, ELISA, Functional Assay, Neutralization
Unconjugated

Details

Human CXCL5
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (0.1 - 0.2 µg/ml), Western blot (0.1 - 0.2 µg/ml), ELISA (0.5 - 1 µg/ml), Functional Assay, Neutralization

Specificity and Use

CXCL5 antibody was raised against e. coli-derived Recombinant, Human CXCL5.
Recognizes human CXCL5. Neutralizes human CXCL5 bioactivity. Based on direct ELISA results, this antibody shows less than 20% cross-reactivity with recombinant, human GCP-2 and less than 5% cross-reactivity with recombinant, mouse KC, recombinant, rat CINC-1, recombinant, human GRO, recombinant, human GRO and recombinant, mouse GCP-2. Additionally, in direct ELISA, this antibody shows no cross-reactivity with other chemokines tested.
Suitable for use in ELISA, Western Blot, Neutralization, Immunohistochemistry. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human CXCL5. The detection limit for Recombinant, Human CXCL5 is approximately 5 ng/lane under non-reducing and reducing conditions. Neutralization of Human CXCL5 Bioactivity. The exact concentration of antibody required to neutralize rhCXCL5 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-human CXCL5 antibody was determined to be approximately 0.2-1 ug/ml in the presence of 30 ng/ml of rhCXCL5, using BaF/3 cells transfected with rhCXCR-2. Direct ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human CXCL5. The detection limit for Recombinant, Human CXCL5 is approximately 0.15 ng/well. Immunohistochemistry: 15 ug/ml Paraffin-embedded tissue sections. To measure the ability of the antibody to neutralize the chemoattractant activity of rhCXCL5 for hCXCR-2 transfected BaF/3cells, Recombinant, Human CXCL5 was incubated with various concentrations of the antibody for 15 minutes at RT in a 96 well microplate. Following this preincubation period, 75 ul of the cytokine-antibody solution (containing rhCXCL5 at a final concentration of 0.03g/ml and antibody at the concentrations indicated) was transferred to the lower compartment of a 96 well chemotaxis chamber (NeuroProbe, Cabin John, MD). The chemotaxis chamber was then assembled using a PVP-free polycarbonate filter (5 micron pore size) and 0.2 x1E6 cells/well was added to the top chamber. After incubation for 3 hours at 37? C in a 5% CO2. humidified incubator, the chamber was carefully disassembled. The cells that migrate through to the lower chamber were transferred to a 96 well plate. Chemotaxis was measured by Resazurin staining of cells that have migrated through the filter. As shown in figure 2, the ND50 for this lot of antibody is approximately 0.2-1 ug/ml.

Packaging

PBS, pH 7.2, 5% trehalose.
Reconstitute with sterile PBS
Lyophilized (short-term) and resuspended forms: -20°C
For research use only.

About CXCL5

P42830 NM_002994 NP_002985.1

CXCL5 Antibody, C-X-C motif chemokine 5 Antibody, ENA-78(1-78) Antibody, GCP-2 Antibody, ENA78 Antibody, SCYB5 Antibody, ENA-78 Antibody, Small inducible cytokine B5 Antibody, Small-inducible cytokine B5 Antibody, LIX Antibody, AMCF-II Antibody

CXCL5 is a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion.

Requested From: United States
Date Requested: 9/27/2016

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