Target
Mouse CSF2 / GM-CSF
Synonyms
CSF2 | Colony-stimulating factor | CSF | GM-CSF | Molgramostin | GMCSF
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Immunogen
Yeast-expressed recombinant mouse GM-CSF.
Specificity
Recognizes mouse Granulocyte Macrophage Colony Stimulating Factor (GM-CSF).
Applications
- Western blot
- ELISA
- Neutralization
Usage
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse GM-CSF protein levels. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse GM-CSF under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing mouse GM-CSF bioactivity. A suitable LE/AF Rat IgG2a should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, No preservatives added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee