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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CREB1 / CREB Antibody (phospho‑Ser121, DY650) LS‑C180290
CREB antibody LS-C180290 is a DY650-conjugated rabbit polyclonal antibody to CREB (CREB1) from human and mouse. Validated for IP and WB.
Catalog
Size
Price
LS-C180290-100
100 µl (0.3 mg/ml)
Unavailable

Popular CREB1 / CREB Products

Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left Lane). The right lane is indicative that the specific immunolabeling of the CREB protein is completely eliminated
Species: Rat, Human, Mouse, Bovine, Dog, Chicken, Xenopus
Applications: Western blot
Antibody
Species: Human, Monkey, Mouse, Rat, Drosophila
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
CREB1 / CREB antibody LS-C110441 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: Western blot
IHC of paraffin-embedded prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

CREB antibody LS-C180290 is a DY650-conjugated rabbit polyclonal antibody to CREB (CREB1) from human and mouse. Validated for IP and WB.
About CREB1 / CREB
CREB (cAMP response element binding protein1), a DNA binding protein, is a member of the leucine zipper family of transcription factors. CREB interaction with DNA requires phosphorylation at Ser 119 by activated cAMP protein kinase A. Once phosphorylated CREB binds to an 8 base enhancer know as the cAMP response element (CRE) containing a conserved core sequence TGACG. CREB also interacts with CBP (CREB binding protein) and RNA polymerase II when phosphorylated on Ser 133. P16220 NM_134442 NP_004370.1

CREB1 Antibody, CREB-1 Antibody, Transactivator protein Antibody, CREB Antibody


Specifications

Target
Human CREB1 / CREB
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
DyLight 650. Also available Unconjugated or conjugated with Biotin, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
CREB1 / CREB antibody was raised against a phospho-peptide spanning the Ser-121 phosphorylation site of human CREB.
Epitope
pSer121
Specificity
The Ser-121-phosphorylated form of CREB.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C in the dark.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with antibody. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H202. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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