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COL4A6 / Collagen IV Antibody (aa1201‑1250) LS‑C119459

Catalog Number Size Price
LS-C119459-50 50 µl (1 mg/ml) $265 
LS-C119459-100 100 µl (1 mg/ml) $325 
Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using Collagen IV alpha6 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Collagen IV alpha6 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using Collagen IV alpha6 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Collagen IV alpha6 Antibody. The picture on the right is blocked with the synthesized peptide.
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COL4A6 / Collagen IV Antibody (aa1201‑1250) LS‑C119459

Antibody:
Rabbit Polyclonal to Human COL4A6 / Collagen IV
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$265 
LS-C119459-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human COL4A6 / Collagen IV
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen IV antibody LS-C119459 is an unconjugated rabbit polyclonal antibody to human Collagen IV (COL4A6 ). Validated for IF, IHC and Peptide-ELISA.
Target
Human COL4A6 / Collagen IV
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence (1:100 - 1:500)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human Collagen IV alpha6. (AA Range: 1201-1250)
Epitope
aa1201-1250
Specificity
Collagen IV alpha6 Antibody detects endogenous levels of total Collagen IV alpha6 protein.
Usage
Western Blot: Predicted MW 163 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COL4A6 / Collagen IV
Q14031 NM_001847 NP_001838.2

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Immunofluorescent analysis of Collagen 4 alpha 6 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Monkey, Rat
Applications: ICC, Immunofluorescence, Western blot
Western blot of COL4A6 antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Human
Range: 0.312-20 ng/ml
Product
Species: Human
Applications: IHC, IHC - Frozen, ICC, Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay

Requested From: United States
Date Requested: 9/20/2019