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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHRNB2 Antibody (aa21‑70) IHC‑plus™ LS‑B937
Note: This antibody replaces LS-C30049
CHRNB2 antibody LS-B937 is an unconjugated rabbit polyclonal antibody to CHRNB2 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B937-50
50 µg (1 mg/ml)
Unavailable

Popular CHRNB2 Products

Antibody
Species: Human
Applications: ICC, Western blot, ELISA
Antibody
Species: Human
Applications: ICC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Anti-CHRNB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8300 dilution 3.75 ug/ml.
Species: Human, Monkey, Bat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

CHRNB2 antibody LS-B937 is an unconjugated rabbit polyclonal antibody to CHRNB2 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CHRNB2
Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. P17787 NM_000748 NP_000739.1

CHRNB2 Antibody, AChR-beta1 Antibody, Acrb2 Antibody, EFNL3 Antibody, NAChR beta 2 Antibody, NAChRB2 Antibody


Specifications

Target
Human CHRNB2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CHRNB2 antibody was raised against synthetic peptide located between aa21-70 of human CHRNB2 (P17787, NP_000739). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Ferret, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Xenopus (100%); Opossum, Sea squirt (92%); Zebra finch, Chicken (85%); Turkey (84%); Pufferfish, Zebrafish (83%).
Blocking Peptide
LS-E10686 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B937. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa21-70
Specificity
Human CHRNB2
Usage
Immunohistochemistry: LS-B937 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B937 was determined to be 10 ug/ml.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CHRNB2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B937 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CHRNB2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B937 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CHRNB2 antibody LS-B937 Western blot of Daudi Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CHRNB2 antibody LS-B937 Western blot of Daudi Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Daudi Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CHRNB2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B937 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CHRNB2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B937 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CHRNB2 antibody LS-B937 Western blot of Daudi Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CHRNB2 antibody LS-B937 Western blot of Daudi Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Daudi Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CHRNB2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B937 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CHRNB2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B937 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CHRNB2 antibody LS-B937 Western blot of Daudi Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CHRNB2 antibody LS-B937 Western blot of Daudi Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Daudi Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CHRNB2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B937 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CHRNB2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B937 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CHRNB2 antibody LS-B937 Western blot of Daudi Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CHRNB2 antibody LS-B937 Western blot of Daudi Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Daudi Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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