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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B8300-50 50 µg (0.5 mg/ml) $425 
CHRNB2 Antibody - Anti-CHRNB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
CHRNB2 Antibody - CHRNB2 antibody (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
CHRNB2 Antibody - CHRNB2 antibody (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CHRNB2 Antibody - Anti-CHRNB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
CHRNB2 Antibody - CHRNB2 antibody (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
CHRNB2 Antibody - CHRNB2 antibody (1 ug/ml) staining of Human Cerebellum lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ Polyclonal CHRNB2 Antibody (aa351‑362, IHC, WB) LS‑B8300

IHCPlus™ Polyclonal CHRNB2 Antibody (aa351‑362, IHC, WB) LS‑B8300

Note: This antibody replaces LS-C112644
Antibody:
Goat Polyclonal to Human CHRNB2
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8300-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human CHRNB2
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bat
Format:
Unconjugated, Unmodified

Specifications

Description
CHRNB2 antibody LS-B8300 is an unconjugated goat polyclonal antibody to CHRNB2 from human, bat and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CHRNB2
Synonyms
CHRNB2 | AChR-beta1 | Acrb2 | EFNL3 | NAChR beta 2 | NAChRB2
Host
Goat
Reactivity
Human, Monkey, Bat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence QPRHHCARQRLR, from the internal region of the protein sequence according to NP_000739.1.
Blocking Peptide
LS-E26678 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8300. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa351-362
Specificity
Human CHRNB2.
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 48 kDa band observed in Human Brain (Cerebellum, Cerebral Cortex) lysates (calculated MW of 57.0 kDa according to NP_000739.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHRNB2
P17787 NM_000748 NP_000739.1

Validation

CHRNB2 Antibody - Anti-CHRNB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-CHRNB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-CHRNB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

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Requested From: United States
Date Requested: 2/27/2020