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Anti-CFL1 / Cofilin Antibody (aa1-166, clone AT1C1) IHC-plus™ LS-B2971

Note: This antibody replaces LS-C93727

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CFL1 / Cofilin Antibodies

Anti-CFL1 / Cofilin Antibody (phospho-Ser3) LS-C35953
Rabbit Monoclonal (IgG) to Human CFL1 / Cofilin
Human, Monkey, Mouse, Rat
Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-CFL1 / Cofilin Antibody (aa1-166, clone AT1C1) IHC-plus™ LS-B2971
Mouse Monoclonal [clone AT1C1] (IgG1,k) to Human CFL1 / Cofilin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CFL1 / Cofilin Antibody (Internal) LS-C358939
Rabbit Polyclonal to Human CFL1 / Cofilin
Human, Mouse, Rat, Pig, Sheep, Zebrafish
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone AT1C1] (IgG1,k) to Human CFL1 / Cofilin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CFL1 / Cofilin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [AT1C1]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA

Specificity and Use

CFL1 / Cofilin antibody was raised against recombinant human Cofilin 1 (1-166aa) purified from E. coli. NCBI Accession No.: NP_005498.
aa1-166
Immunohistochemistry: LS-B2971 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2971 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CFL1 / Cofilin

P23528 NM_005507 NP_005498.1

CFL1 Antibody, 18 kDa phosphoprotein Antibody, Cofilin Antibody, CFL Antibody, Cofilin 1 (non-muscle) Antibody, Cofilin, non-muscle isoform Antibody, p18 Antibody

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.

Immunohistochemistry

Immunohistochemistry
Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2971 concentration 10 ug/ml.

Western blot

Western blot
The lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/8/2016

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