Target
Human CENPA / CENP-A
Synonyms
CENPA | Centromere autoantigen A | Centromere-specific histone | CenH3 | Centromere protein A | Centromere protein A, 17kDa | CENP-A | Centromere protein A (17kD)
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A. KLH conjugated. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%); Gibbon, Rabbit (93%); Nectri (86%).
Specificity
Recognizes human CENP-A, Mr17kD. Broad species cross-reactivity predicted due to sequence conservation.
Usage
Suitable for use in Western Blot analysis. Western Blot Analysis: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa acid extracted histones. 20 ug control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2 ug/ml anti-CENP-A. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 5 mg/ml BSA, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee