CENP-A antibody LS-C80799 is an unconjugated rabbit polyclonal antibody to CENP-A (CENPA) from human, mouse, rat and other species. Validated for WB.
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit, Zebrafish
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
CENPA / CENP-A antibody was raised against synthetic peptide from C-Terminus of human CENPA (P49450, NP_001800). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%); Elephant, Pig, Zebra finch, Lizard (92%); Mouse (85%); Opossum, Chicken, Platypus, Salmon, Stickleback, Pike, Zebrafish, Arabidopsis (84%).
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.