Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat, Pig (tested or 100% immunogen sequence identity)
Protein A purified
Immunoprecipitation (1:3 - 1:10)
Specificity and Use
CDK4 antibody was raised against human recombinant full-length cdk4 polypeptide.
Species cross-reactivity: Human, porcine, rat, mouse. Reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle; it does not cross-react with cyclins. Especially suitable for immunoprecipitation, Mab precipitates the cdk4/cyclin complex.
Suitable for Immunoprecipitation and Western Blot (Western). Immunoprecipitation: 1:3-1:10; Incubation Time 1-4 hr at RT or overnight at 2-8?C.
50 µl deionized water
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase.