The Immunohistochemistry Antibody Company
|Human, Monkey, Rat, Rabbit, Sheep (tested or 100% immunogen sequence identity)|
|Mouse, Bovine, Dog, Guinea pig, Hamster, Horse, Pig (at least 90% immunogen sequence identity)|
|CDK4 antibody was raised against synthetic peptide (C)NPHKRISAFRALQHSYLHKDEG based on the human Cdk4 (residues 279-300) with the cysteine (C) residue added and the peptide coupled to KLH. The amino acid sequence differs from pig by 1 a. a., from mouse by 2 a. a., and from rat by 3 a. a. over these residues. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%); Galago, Bovine, Pig, Guinea pig (95%); Mouse, Dog, Hamster, Panda, Horse (91%); Rat (86%).|
|Detects an ~34 kD protein, corresponding to the apparent molecular mass of Cdk4 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, pig (weak), rabbit (weak) and sheep origins.|
|Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.|
|For research use only.|
CDK4 Antibody, Cell division protein kinase 4 Antibody, Crk3 Antibody, Cyclin-dependent kinase 4 Antibody, CMM3 Antibody, p34/cdk4 Antibody, PSK-J3 AntibodySer/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase.