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Anti-CDK2 Antibody (Thr160) IHC-plus™ LS-B1464

Note: This antibody replaces LS-C34337, LS-C78979, LS-C51485

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

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Rabbit Polyclonal (IgG) to Human CDK2
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human CDK2
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot

Specificity and Use

CDK2 antibody was raised against synthetic peptide from human CDK2.
Thr160
Amino acids surrounding Thr 160 of human CDK2
Immunohistochemistry: LS-B1464 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1464 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide, 50% glycerol.
Long term: -70°C; Short term: -20°C
For research use only.

About CDK2

P24941 NM_001798 NP_001789.2

CDK2 Antibody, Cell division protein kinase 2 Antibody, CDC2-related protein kinase Antibody, p33 protein kinase Antibody, p33(CDK2) Antibody, CDKN2 Antibody, Cyclin-dependent kinase 2 Antibody

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA.

Immunohistochemistry

Immunohistochemistry
Anti-CDK2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1464 concentration 5 ug/ml.

Western blot

Western blot
Western blot of extracts from 293, MCF, 3T3 cells using CDK2(Ab-160) antibody.

Requested From: 
Date Requested: 4/24/2017

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