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Anti-CDK2 Antibody (phospho-Thr160) LS-C5980

Ordering

Wt. Vol. Conc. Price
- 100 µl - $485
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rabbit Polyclonal (IgG) to Human CDK2
Human, Mouse, Rat
Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human CDK2
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified

Applications

Western blot (1:1000), Immunoprecipitation (1:150), ELISA

Specificity and Use

CDK2 antibody was raised against synthetic phosphopeptide corresponding to residues surrounding Thr160 of human cdk2 (KLH coupled).
pThr160
Detects cdk2 only when phosphorylated at threonine 160. Weakly cross-reacts with cdc2 phosphorylated at Thr161. Does not cross-react with other phosphorylated cyclin-dependent kinases. Species cross-reactivity: Human, mouse and rat.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:150.

Packaging

10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CDK2

P24941 NM_001798 NP_001789.2

CDK2 Antibody, Cell division protein kinase 2 Antibody, CDC2-related protein kinase Antibody, p33 protein kinase Antibody, p33(CDK2) Antibody, CDKN2 Antibody, Cyclin-dependent kinase 2 Antibody

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA.

Requested From: United States
Date Requested: 9/28/2016

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