Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
CDC25C, a CDC25 dual specificity protein phosphatase, has been shown to affect the regulation of cell cycle control, and specifically the G2/M progression, through interaction with cdc/cyclin kinases and proliferating cell nuclear antigen (PCNA). Unlike CDC25A and CDC25B, CDC25C does not seem to have oncogenic properties. CDC25C null mutant mice are viable, fertile, and do not display obvious abnomalities.
Immunoperoxidase of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6 ug/ml]
CDC25C monoclonal antibody (M01), clone 3B11 Western blot of CDC25C expression in HeLa.
Western blot of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.
Immunoprecipitation of CDC25C transfected lysate using anti-CDC25C monoclonal antibody and Protein A Magnetic Bead.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged CDC25C is approximately 0.1 ng/ml as a capture antibody.
Western blot of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody, clone 3B11. GAPDH ( 36.1 kD ) used as specificity and loading control.