Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone 53-6-7] (IgG2a,k) to Mouse CD8A / CD8 Alpha
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Mouse Monoclonal (IgG1) to Rat CD8A / CD8 Alpha
Rat CD8A / CD8 Alpha
Rat (tested or 100% immunogen sequence identity)
Ion exchange chromatography
Flow Cytometry (1:5 - 1:10)
Specificity and Use
Rat thymocyte membrane glycoproteins.
Recognizes rat CD8 cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.
Suitable for use in Flow cytometry: 1:5-1:10; 10 ul labels 10^6 cells in 100ul. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.4, 1% BSA, 0.09% sodium azide
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. Sensitive to light.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain.