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Catalog Number Size Price
LS-B14771-50 50 µg (1 mg/ml) $460 
CD8A / CD8 Alpha Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD8A / CD8 Alpha Antibody - IHC testing of FFPE tonsil tissue with CD8 antibody (clone C8/144B).  This image was taken for the unmodified form of this product. Other forms have not been tested.
CD8A / CD8 Alpha Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD8A / CD8 Alpha Antibody - IHC testing of FFPE tonsil tissue with CD8 antibody (clone C8/144B).  This image was taken for the unmodified form of this product. Other forms have not been tested.
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IHC‑plus™ Monoclonal Mouse anti‑Human CD8A / CD8 Alpha Antibody (clone C8/144B, Azide‑free,BSA‑free, C‑Terminus, IHC, IF) LS‑B14771

IHC‑plus™ Monoclonal Mouse anti‑Human CD8A / CD8 Alpha Antibody (clone C8/144B, Azide‑free,BSA‑free, C‑Terminus, IHC, IF) LS‑B14771

Note: This antibody replaces LS-C389847
Antibody:
CD8A / CD8 Alpha Mouse anti-Human Monoclonal (C-Terminus) (Azide-free, BSA-free) (C8/144B) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Price
Catalog Number
$460
LS-B14771-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD8A / CD8 Alpha Mouse anti-Human Monoclonal (C-Terminus) (Azide-free, BSA-free) (C8/144B) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:

Specifications

Description
CD8 Alpha antibody LS-B14771 is an unconjugated mouse monoclonal antibody to human CD8 Alpha (CD8A) (C-Terminus). Validated for Flow, IF and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human CD8A / CD8 Alpha
Synonyms
CD8A | CD8a molecule | CD8a antigen | OKT8 T-cell antigen | Leu2 T-lymphocyte antigen | T8 T-cell antigen | T cell co-receptor | p32 | CD8 | Leu2 | T-cell antigen Leu2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
C8/144B
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Azide-free, BSA-free. Also available Unmodified or Ready-to-Use.
Immunogen
13 AA peptide from the C-Terminus cytoplasmic domain of the alpha chain.
Epitope
C-Terminus
Specificity
Human CD8A / CD8 Alpha
Applications
  • IHC
  • IHC - Paraffin (1 - 5 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. CD8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD8 and 4 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8a antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral Tcell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+/CD8-, and in T-lymphoblastic lymphoma/leukemia, they are often co-expressed. It is also found in littoral cell angioma of the spleen. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
1X PBS
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD8A / CD8 Alpha
P01732 NM_001768 NP_001759.3

Validation

CD8A / CD8 Alpha Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ CD8A / CD8 Alpha Antibody (C-Terminus, clone C8/144B, Azide-free, BSA-free) for IHC, IF/Immunofluorescence, Flow LS-B14771 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/8/2024