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Anti-CD86 Antibody (clone GL1, PE, Cy5) LS-C107447

Ordering

Wt. Vol. Conc. Price
50 µg - - $265
100 µg - - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD86 Antibodies

Anti-CD86 Antibody (clone BU63, FITC) LS-C112510
Mouse Monoclonal [clone BU63] (IgG1) to Human CD86
Human
Flow Cytometry
FITC Conjugated
Anti-CD86 Antibody IHC-plus™ LS-B11911
Rabbit Polyclonal (IgG) to Human CD86
Human, Mouse
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone GL1] (IgG2a,k) to Mouse CD86
Mouse
Flow Cytometry
PE, Cy5 Conjugated

Details

Mouse CD86
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [GL1]
Phycoerythrin, Cy5
Affinity purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

CD86 antibody was raised against mouse CD86
The GL1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.06 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
Store at +4°C. Do not freeze. Product is photosensitive and should be protected from light.
For research use only.

About CD86

P42081 NM_006889 NP_008820.3

CD86 Antibody, Activation B7-2 antigen Antibody, B7-2 Antibody, B7.2 Antibody, B70 Antibody, CD86 molecule Antibody, CTLA-4 counter-receptor B7.2 Antibody, CD86 antigen Antibody, FUN-1 Antibody, LAB72 Antibody, BU63 Antibody, CD28LG2 Antibody, Ctla-4 counter-receptors b7.2 Antibody

CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. It is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell.

Flow Cytometry

Flow Cytometry
Staining of 3-day LPS activated BALB/c splenocytes with 0.03 ug of PE-Cy5 Rat IgG2a isotype control (open histogram) or PE-Cy5 anti-mouse CD86 (GL1) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/7/2016

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