Human CD86

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

CDLA86

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

Recombinant human full-length protein.

Human CD86

• IHC - Paraffin (2 - 4 µg/ml)
• Western blot (0.5 - 1 µg/ml)

CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is a receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt] Optimal dilution of the CD86 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Short Term: Store at 2°C to 8°C. Long Term: At -20°C. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P42081 NCBI: NM_006889 NP_008820.3