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Anti-CD45 / LCA Antibody (clone YW62.3, RPE) LS-C43236

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Wt. Vol. Conc. Price
- 500 tst - $405
Inquire for larger quantities

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Most Popular CD45 / LCA Antibodies

Anti-CD45 / LCA Antibody (PE) LS-C127700
Mouse Monoclonal (IgG1) to Sheep CD45 / LCA
Sheep, Bovine, Goat
Flow Cytometry
PE Conjugated
Anti-CD45 / LCA Antibody (Internal) IHC-plus™ LS-A9435
Rabbit Polyclonal to Human CD45 / LCA
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone YW62.3] (IgG2b) to Mouse CD45 / LCA
Mouse
Flow Cytometry
RPE Conjugated

Details

Mouse CD45 / LCA
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [YW62.3]
R. Phycoerythrin
Protein G purified
Unmodified

Applications

Flow Cytometry (1:1)

Specificity and Use

CD45 / LCA antibody was raised against mouse spleen cells.
Recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kD glycoprotein expressed by all leucocytes. CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains, this was subsequently expanded to include a third allele encoding Ly5.3. Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1. Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb;. A number of different isoforms isoforms of CD45 are expressed on murine leucocytes depending on the pattern of alternative splicing of 3 exons termed A, B and C encoding regions of ~50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. Is reactive with all isoforms of murine CD45. N. B. Some reactivity with human tissue has been observed.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized, PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
500 tst Distilled Water.
For research use only.

About CD45 / LCA

P08575 NM_002838 NP_002829.3

PTPRC Antibody, B220 Antibody, CD45R Antibody, CD45 Antibody, gp180 Antibody, Leukocyte common antigen Antibody, L-CA Antibody, Leucocyte common antigen Antibody, LY5 Antibody, T200 Antibody, T200 glycoprotein Antibody, T200 leukocyte common antigen Antibody, CD45 antigen Antibody, LCA Antibody

CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP.

Requested From: United States
Date Requested: 12/3/2016

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