LSBio The Immunohistochemistry Antibody Company
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-CD40L Antibody LS-C150170


Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular CD40L Antibodies

Anti-CD40L Antibody (N-Terminus) LS-C121522
Rabbit Polyclonal (IgG) to Human CD40L
IHC - Paraffin, Western blot
Anti-CD40L Antibody IHC-plus™ LS-B7421
Goat Polyclonal (IgG) to Human CD40L
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Mouse CD40L
ICC, Western blot, ELISA, Neutralization


Mouse CD40L
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • ICC (15 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • Neutralization

Specificity and Use

CD40L antibody was raised against nS0-derived rmCD40 Ligand (aa 61 - 260).
Mouse CD40 Ligand.
Neutralization of Mouse CD40 Ligand Bioactivity-The exact concentration of antibody required to neutralize rmCD40 Ligand activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CD40 Ligand antibody was determined to be approximately 0.2-0.6 µg/ml in the presence of 100 ng/ml of rmCD40 Ligand using mouse splenic B-cells. Western blot-This antibody can be used at 0.1-0.2 µg/ml with the appropriate secondary reagents to detect mouse CD40 Ligand. The detection limit for rmCD40 Ligand is approximately 2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry-This antibody will detect CD40 Ligand in cells. The working dilution is 15 µg/ml. ELISA Capture-This antibody can be used as a capture antibody in a mouse CD40 Ligand ELISA in combination with biotinylated, mouse CD40 Ligand affinity purified polyclonal detection antibody. Using plates coated with 100 ul/well of the capture antibody at. 0.8 µg/ml, in combination with 100 ul/well of the detection antibody can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 6 ng/ml.


Lyophilized from 0.2 µm filtered 100 mM NaHCO3, pH 8.3, 500 mM NaCl, 1% sucrose.
Store at -20°C.
For research use only.

About CD40L

P29965 NM_000074 NP_000065.1

CD40LG Antibody, CD154 Antibody, CD154 antigen Antibody, CD40-L Antibody, CD40 ligand Antibody, CD40L Antibody, gp39 Antibody, HIGM1 Antibody, IGM Antibody, IMD3 Antibody, T-BAM Antibody, TNFSF5 Antibody, CD40 antigen ligand Antibody, CD54 Antibody, HCD40L Antibody, T-B cell-activating molecule Antibody, T-cell antigen Gp39 Antibody, TNF-related activation protein Antibody

TNFSF5 is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in inability of immunoglobulin class switch and is associated with hyper IgM syndrome.

Requested From: United States
Date Requested: 10/22/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number