CD34 antibody LS-B2652 is an unconjugated mouse monoclonal antibody to human CD34. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Western blot
- Flow Cytometry
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CD34 antibody was raised against human KG-1a tumor cells.
Recognizes a class 2 epitope of the 110kD human CD34 molecule.
Immunohistochemistry: LS-B2652 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2652 was determined to be 10 ug/ml.
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for up to 1 year.
For research use only.