(tested or 100% immunogen sequence identity)
- IHC - Frozen
- Flow Cytometry
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CD22 antibody was raised against human tonsil lymphocytes
The CD22 antigen is a heterodimer composed of two glycoprotein chains of 130-140 kD and is present as both a cytoplasmic and membrane antigen on B lymphocytes. Antigen expression appears at an early stage in B lymphocyte differentiation at approximately the same stage as the CD19 antigen. Surface expression of the CD22 antigen by B lymphocytes is variable. Circulating B lymphocytes are CD22 positive and follicular mantle and marginal zone B lymphocytes show strong binding of this antibody. Germinal centre B cells exhibit only weak expression of the CD22 antigen. The CD22 antigen is poorly expressed by non T ALL cells and by a proportion of B CLL cells. Hairy cell leukaemia cells have been demonstrated to strongly express the CD22 antigen. CD22 functions as a sialic acid binding lectin specifically sialic acid in alpha-2,6 linkage. Sialylation of CD22 by alpha-2,6 sialyl transferase inhibits CD22 ligand binding. Antigen distribution: B cells (lg+, E-) 92+/- 3%; Peripheral blood lymphocytes 12 +/- 3%; Thymocytes < 1%; Granulocytes < 1%; Monocytes >1%T cells (E+) >1%
Identification of B lymphocytes and HCL cells by indirect immunofluorescence staining. Identification of B lymphocytes and B lymphocyte progenitor cells by staining of cytoplasmic CD22 antigen using the APAAP technique. Staining of B lymphocytes in frozen
PBS, 10 mM sodium azide, 1 mg/ml BSA
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
For research use only.
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