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Catalog Number Size Price
LS-C407903-10 10 µg $318 
LS-C407903-100 100 µg $470 
CD22 Antibody - CD22 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
CD22 Antibody - CD22 antibody Western blot. All lanes: Anti CD22 at 0.5 ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40 ug. Lane 2: 22RV1 Whole Cell Lysate at 40 ug. Predicted band size: 95 kD. Observed band size: 95 kD.
CD22 Antibody - Flow Cytometry analysis of Raji cells using anti-CD22 antibody. Overlay histogram showing Raji cells stained with anti-CD22 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD22 Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CD22 Antibody - CD22 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
CD22 Antibody - CD22 antibody Western blot. All lanes: Anti CD22 at 0.5 ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40 ug. Lane 2: 22RV1 Whole Cell Lysate at 40 ug. Predicted band size: 95 kD. Observed band size: 95 kD.
CD22 Antibody - Flow Cytometry analysis of Raji cells using anti-CD22 antibody. Overlay histogram showing Raji cells stained with anti-CD22 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD22 Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human CD22 Antibody (aa696‑724, IHC, WB) LS‑C407903

Polyclonal Rabbit anti‑Human CD22 Antibody (aa696‑724, IHC, WB) LS‑C407903

Antibody:
CD22 Rabbit anti-Human Polyclonal (aa696-724) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407903-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD22 Rabbit anti-Human Polyclonal (aa696-724) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD22 antibody LS-C407903 is an unconjugated rabbit polyclonal antibody to human CD22 (aa696-724). Validated for IHC and WB.
Target
Human CD22
Synonyms
CD22 | CD22 molecule | CD22 antigen | SIGLEC2 | SIGLEC-2 | T-cell surface antigen Leu-14 | B-cell receptor CD22 | BL-CAM
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human CD22 (696-724 aa LAILILAICGLKLQRRWKRTQSQQGLQEN), different from the related mouse sequence by ten amino acids.
Epitope
aa696-724
Specificity
B-lymphocytes.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD22
P20273 NM_001771 NP_001762.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2024