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Anti-CD22 Antibody LS-C104507

Note: This antibody replaces LS-C35475

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD22 Antibodies

Anti-CD22 Antibody LS-C4802
Mouse Monoclonal (IgG1) to Human CD22
Human
IHC - Paraffin
Unconjugated
Anti-CD22 Antibody (clone 4C3) LS-C174778
Mouse Monoclonal [clone 4C3] (IgG1) to Human CD22
Human, Monkey, Mouse, Rat, Dog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CD22 Antibody (C-Terminus, clone SP104) LS-C210357
Rabbit Monoclonal [clone SP104] (IgG) to Human CD22
Human
IHC - Paraffin, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CD22
Human
Western blot, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human CD22
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

CD22 antibody was raised against cHO cell derived recombinant Human sCD22.
Human sCD22
ELISA: To detect hCD22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CD22 (LS-C104523) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22. Western Blot: To detect hCD22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCD22 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About CD22

P20273 NM_001771 NP_001762.2

CD22 Antibody, CD22 molecule Antibody, CD22 antigen Antibody, SIGLEC2 Antibody, SIGLEC-2 Antibody, T-cell surface antigen Leu-14 Antibody, B-cell receptor CD22 Antibody, BL-CAM Antibody

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling.

Western blot

Western blot
Western Blot (non-reducing) of CD22 antibody LS-C104507. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of CD22 antibody LS-C104507. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/2/2016

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