Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
IHC - Paraffin (1:125)
Western blot (1:500)
Flow Cytometry (1:20)
Specificity and Use
CCR2 antibody was raised against synthetic peptide SHSLFTRSIQELDEGATTPYDYDDGEPC corresponding to aa 18-45 of the N-terminal domain of mouse CCR2. Percent identity by BLAST analysis: Mouse (100%); Rat (96%); Hamster (82%).
This antibody recognizes mouse CCR2 and the peptide sequence is <50 % identical to other chemokine receptors in this region.
Immunocytochemistry (ICC) (acetone fixed cells)
10 mM KHPO4, 140 mM NaCl with 0.1% sodium azide and 1 mg/ml BSA
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.
CCR2, a Chemokine Receptor, is a regulator of monocyte/macrophage trafficking and is upregulated in response to inflammation. Resistance to HIV-1 infection or delayed progression to AIDS may be linked to CCR2 polymorphisms. The ligand for CCR2 is monocyte chemoattractant protein (MCP-1), a chemokine produced by endothelial cells, smooth muscle cells, and macrophages.