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Anti-CCND1 / Cyclin D1 Antibody (Ser90) IHC-plus™ LS-B3452

Note: This antibody replaces LS-C98547


Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $395

LSBio (Direct) LSBio (Direct)

CCND1 / Cyclin D1 Antibody LS-B3452 Images

[click image for a larger image and more details]
Anti-CCND1 / Cyclin D1 antibody IHC of human testis.
Confocal immunofluorescent of Cyclin D1 Antibody (S90) with HeLa cell followed by Alexa Fluor...
Western blot
Western blot of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35 ug/lane).

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Rabbit Polyclonal to Human CCND1 / Cyclin D1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot


Human CCND1 / Cyclin D1
Human, Mouse (tested or 100% immunogen sequence identity)
Immunoaffinity purified


IHC - Paraffin (10 µg/ml), Immunofluorescence (1:10 - 1:50), Western blot (1:1000)

Specificity and Use

CCND1 / Cyclin D1 antibody was raised against kLH conjugated synthetic peptide corresponding to amino acid residues surrounding S90 of human Cyclin D1.
LS-E7012 - Lyophilized - 100 µg - $145.00
Immunohistochemistry: LS-B3452 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3452 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not yet been evaluated for use in IHC with mouse samples.


PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About CCND1 / Cyclin D1

P24385 NM_053056 NP_444284.1

CCND1 Antibody, B-cell lymphoma 1 protein Antibody, BCL-1 oncogene Antibody, B-cell CLL/lymphoma 1 Antibody, BCL-1 Antibody, Cyclin D1 Antibody, G1/S-specific cyclin-D1 Antibody, U21B31 Antibody, PRAD1 Antibody, BCL1 Antibody, D11S287E Antibody, PRAD1 oncogene Antibody

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase.


Anti-CCND1 / Cyclin D1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3452 concentration 10 ug/ml.


Confocal immunofluorescent of Cyclin D1 Antibody (S90) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Western blot

Western blot
Western blot of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35 ug/lane). Cyclin D1(arrow) was detected using the purified antibody.

Requested From: United States
Date Requested: 10/8/2015

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