Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Delipidated and defibrinated
Western blot (1:500 - 1:1000)
ELISA (1:2000 - 1:10000)
IHC - Paraffin
Specificity and Use
Full length synthetic polypeptide corresponding to the human gene sequence.
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kD in size corresponding to Cyclin D1 by western blotting in the appropriate cell lysate or extract. Anti-Cyclin D1 is suitable for the detection by immunoblot of human, rat and mouse Cyclin D1.
0.02M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase.
Western blot analysis is shown using Anti-Cyclin D1 antibody to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kD corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10000 of HRP conjugated Go.