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Anti-CCL25 / TECK Antibody LS-C104396

Note: This antibody replaces LS-C53703, LS-C187922, LS-C18049

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCL25 / TECK Antibodies

Anti-CCL25 / TECK Antibody LS-C18050
Mouse Monoclonal (IgG1,k) to Human CCL25 / TECK
Human
ELISA
Unconjugated
Anti-CCL25 / TECK Antibody LS-C104396
Rabbit Polyclonal to Human CCL25 / TECK
Human
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-CCL25 / TECK Antibody LS-C104428
Mouse Monoclonal (IgG1) to Human CCL25 / TECK
Human
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CCL25 / TECK
Human
IHC, Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Unconjugated

Details

Human CCL25 / TECK
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (10 - 15 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

CCL25 / TECK antibody was raised against e.coli derived recombinant Human TECK.
Human TECK
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 0.5 ug/ml-1.0ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hTECK (100 ng/ml), a concentration of 10-15 ug/ml of this antibody is required. ELISA: To detect hTECK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TECK (LS-C104509) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTECK. Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About CCL25 / TECK

O15444 NM_005624 NP_005615.2

CCL25 Antibody, C-C motif chemokine 25 Antibody, Ck beta-15 Antibody, TECK Antibody, Thymus-expressed chemokine Antibody, Small-inducible cytokine A25 Antibody, TECKvar Antibody, Chemokine TECK Antibody, Ckb15 Antibody, SCYA25 Antibody, Thymus expressed chemokine Antibody

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Immunohistochemistry

Immunohistochemistry
IHC of TECK / CCL25 antibody LS-C104396. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

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