LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
CCL25 / TECK Antibody LS‑C104396
Note: This antibody replaces LS-C53703, LS-C187922, LS-C18049
Antibody
TECK antibody LS-C104396 is an unconjugated rabbit polyclonal antibody to human TECK (CCL25). Validated for ELISA, Neut and WB.
Catalog
Size
Price
LS-C104396-50
50 µg
Unavailable
LS-C104396-100
100 µg
Unavailable

Popular CCL25 / TECK Products

Antibody
Species: Human
Applications: ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA, Neutralization
Western blot of extracts of various cell lines, using LGALS3 antibody.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot

Product Description

TECK antibody LS-C104396 is an unconjugated rabbit polyclonal antibody to human TECK (CCL25). Validated for ELISA, Neut and WB.
About CCL25 / TECK
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. O15444 NM_005624 NP_005615.2

CCL25 Antibody, C-C motif chemokine 25 Antibody, Ck beta-15 Antibody, TECK Antibody, Thymus-expressed chemokine Antibody, Small-inducible cytokine A25 Antibody, TECKvar Antibody, Chemokine TECK Antibody, Ckb15 Antibody, SCYA25 Antibody, Thymus expressed chemokine Antibody


Specifications

Target
Human CCL25 / TECK
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (10 - 15 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CCL25 / TECK antibody was raised against e.coli derived recombinant Human TECK.
Specificity
Human TECK
Usage
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 0.5 ug/ml-1.0ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hTECK (100 ng/ml), a concentration of 10-15 ug/ml of this antibody is required. ELISA: To detect hTECK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TECK (LS-C104509) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTECK. Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Requested From: 
Date Requested: 6/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number