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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CBFA1 / RUNX2 Antibody (DY650) LS‑C180751
RUNX2 antibody LS-C180751 is a DY650-conjugated rabbit polyclonal antibody to RUNX2 (CBFA1) from human and mouse. Validated for ICC and IHC.
Catalog
Size
Price
LS-C180751-100
100 µl (1 mg/ml)
Unavailable

Popular CBFA1 / RUNX2 Products

Western blot of human RUNX2in Saos-2 cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at2 ug/ml.
Species: Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Pig, Chicken
Applications: Western blot
Anti-RUNX2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4293 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-RUNX2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4294 concentration 5 ug/ml.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-RUNX2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4296 concentration 5 ug/ml.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
IHC of RUNX-2 (D198) pAb in paraffin-embedded human placenta tissue.
Species: Human, Mouse, Rat
Applications: Western blot

Product Description

RUNX2 antibody LS-C180751 is a DY650-conjugated rabbit polyclonal antibody to RUNX2 (CBFA1) from human and mouse. Validated for ICC and IHC.
About CBFA1 / RUNX2
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Q13950 NM_004348 NP_004339.3

RUNX2 Antibody, AML3 Antibody, CCD Antibody, CCD1 Antibody, OSF2 Antibody, PEBP2A1 Antibody, PEBP2A2 Antibody, PEA2-alpha A Antibody, PEBP2-alpha A Antibody, OSF-2 Antibody, PEBP2A Antibody, PEBP2aA1 Antibody, CBF-alpha-1 Antibody, CBFA1 Antibody, Oncogene AML-3 Antibody, PEA2aA Antibody, PEBP2aA Antibody


Specifications

Target
Human CBFA1 / RUNX2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
DyLight 650. Also available Unconjugated or conjugated with Biotin, DY488, DY550.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • ICC (1:100)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CBFA1 / RUNX2 antibody was raised against a synthetic peptide made to an internal region of the human RUNX2 protein (within residues 300-375). [Swiss-Prot Q13950].
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C in the dark.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: RUNX2 Antibody - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: RUNX2 Antibody - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: RUNX2 Antibody - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: RUNX2 Antibody - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: RUNX2 Antibody - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: RUNX2 Antibody - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: RUNX2 Antibody - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: RUNX2 Antibody - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: RUNX2 Antibody - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: RUNX2 Antibody - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: RUNX2 Antibody - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: RUNX2 Antibody - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry: RUNX2 Antibody - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry: RUNX2 Antibody - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Immunocytochemistry/Immunofluorescence: RUNX2 Antibody - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunocytochemistry/Immunofluorescence: RUNX2 Antibody - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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