Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1D8] (IgG2b,k) to Human CBFA1 / RUNX2
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Human CBFA1 / RUNX2
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [1D8]
Protein A purified
IHC - Paraffin (5 µg/ml)
Immunofluorescence (10 µg/ml)
Specificity and Use
CBFA1 / RUNX2 antibody was raised against rUNX2 (NP_004339, 251 a.a. ~ 351 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control SJCRH30. Western Blot (Cell lysate) - positive control K-562. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
PBS, pH 7.4. Sourced in Ascites.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters.