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Antibodies > CAST / Calpastatin > LS-C661953
 
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Catalog Number Size Price
LS-C661953-10 10 µg $318 
LS-C661953-100 100 µg $470 
CAST / Calpastatin Antibody - Calpastatin was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- Calpastatin Antigen Affinity purified polyclonal antibody
CAST / Calpastatin Antibody - Calpastatin was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- Calpastatin Antigen Affinity purified polyclonal antibody
CAST / Calpastatin Antibody - Western blot - Anti-Calpastatin Picoband Antibody
CAST / Calpastatin Antibody - Flow Cytometry analysis of U20S cells using anti-Calpastatin antibody. Overlay histogram showing U20S cells stained with anti-Calpastatin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CAST / Calpastatin Antibody - Flow Cytometry analysis of A431 cells using anti-Calpastatin antibody. Overlay histogram showing A431 cells stained with anti-Calpastatin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CAST / Calpastatin Antibody - Flow Cytometry analysis of A549 cells using anti-Calpastatin antibody. Overlay histogram showing A549 cells stained with anti-Calpastatin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CAST / Calpastatin Antibody - Calpastatin was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- Calpastatin Antigen Affinity purified polyclonal antibody
CAST / Calpastatin Antibody - Calpastatin was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- Calpastatin Antigen Affinity purified polyclonal antibody
CAST / Calpastatin Antibody - Western blot - Anti-Calpastatin Picoband Antibody
CAST / Calpastatin Antibody - Flow Cytometry analysis of U20S cells using anti-Calpastatin antibody. Overlay histogram showing U20S cells stained with anti-Calpastatin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CAST / Calpastatin Antibody - Flow Cytometry analysis of A431 cells using anti-Calpastatin antibody. Overlay histogram showing A431 cells stained with anti-Calpastatin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CAST / Calpastatin Antibody - Flow Cytometry analysis of A549 cells using anti-Calpastatin antibody. Overlay histogram showing A549 cells stained with anti-Calpastatin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human CAST / Calpastatin Antibody (IHC, WB) LS‑C661953

Polyclonal Rabbit anti‑Human CAST / Calpastatin Antibody (IHC, WB) LS‑C661953

Submit Review LSBio Guarantee
Antibody:
CAST / Calpastatin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661953-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CAST / Calpastatin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Calpastatin antibody LS-C661953 is an unconjugated rabbit polyclonal antibody to human Calpastatin (CAST). Validated for Flow, ICC, IHC and WB.
Target
Human CAST / Calpastatin
Synonyms
CAST | Calpastatin | BS-17 | Calpain inhibitor | Sperm BS-17 component
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Calpastatin (275-310 aa QEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLR), different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by eleven amino
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CAST / Calpastatin
P20810 NM_001750 NP_001741.4

Publications (0)

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Customer Reviews (0)

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Species: Human
Applications: IHC, IHC - Frozen, Western blot, ELISA
CAST / Calpastatin Antibody - Immunohistochemistry of paraffin-embedded human kidney using CAST / Calpastatin antibody at dilution of 1:10. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CAST / Calpastatin Antibody (Biotin) LS‑C317747
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
CAST / Calpastatin ELISA Kit
Bovine CAST / Calpastatin (Sandwich ELISA) ELISA Kit - LS-F16388
Reactivity:
Range: 0.156-10 ng/ml
Product
Human CAST / Calpastatin Protein (Recombinant 6His, N-terminus) (Full Length) - LS-G20563
Source: E. coli
Tag: 6His, N-terminus
AXL Antibody - Anti-AXL antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC‑plus™ AXL Antibody (clone 7E10) LS‑B6124
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
See all CAST / Calpastatin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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