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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CAST / Calpastatin

Calpastatin

CAST / Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.

Gene Name: Calpastatin
Synonyms: CAST, Calpastatin, BS-17, Calpain inhibitor, Sperm BS-17 component
Target Sequences: NM_001750 NP_001741.4 P20810

Filtered By: None
Target
CAST / Calpastatin (30)
Reactivity
Human (28)
Mouse (5)
Rat (4)
Bovine (2)
Application
IHC (16)
IHC-Fr (3)
IHC-P (9)
WB (30)
Flo (3)
ELISA (11)
ICC (4)
IF (5)
IP (1)
Host
rabbit (22)
mouse (7)
chicken (1)
Isotype
IgG (19)
IgG1 (4)
IgG1,k (1)
IgG2a (1)
Clonality
monoclonal mc (6)
polyclonal pc (24)
Clone
1E7 (1)
1F7E3D10 (1)
2C5 (1)
2G11D6 (1)
CSL1-5 (1)
Format
AP Conjugated (1)
APC Conjugated (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (21)
Epitope
aa1-667 (1)
Publications
No (30)
Showing 1-25 of 30 results

CAST / Calpastatin Antibody (aa1-667) IHC-plus™ LS-B13796
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $425.00
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CAST / Calpastatin Antibody (HRP) LS-C317751
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Human
ELISA IHC IHC-P WB
HRP Conjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human prostate using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human kidney using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human prostate using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human kidney using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CAST / Calpastatin Antibody (Biotin) LS-C317747
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Human
ELISA IHC IHC-P WB
Biotin Conjugated
50µg  $285.00; 100µg 0.25mg/ml  $385.00
Immunohistochemistry of paraffin-embedded human prostate using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human kidney using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human prostate using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human kidney using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CAST / Calpastatin Antibody (FITC) LS-C317754
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Human
ELISA IHC IHC-P WB
FITC Conjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human prostate using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human kidney using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human prostate using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry of paraffin-embedded human kidney using CAST / Calpastatin antibody at dilution of 1:10.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CAST / Calpastatin Antibody LS-C96171
Chicken Polyclonal to Human CAST / Calpastatin
Human
ELISA WB
Unconjugated
100µg 100µl  $365.00

CAST / Calpastatin Antibody (clone 1E7) LS-C337646
Mouse Monoclonal [clone 1E7] (IgG1) to Human CAST / Calpastatin
Human
Flo IHC IHC-P WB
Unconjugated
100µl 0.62mg/ml  $345.00
IHC of paraffin-embedded Human colon tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Human colon tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

CAST / Calpastatin Antibody LS-C661953
Rabbit Polyclonal to Human CAST / Calpastatin
Human
Flo ICC IHC IHC-Fr IHC-P WB
Unconjugated
100µg  $355.00
Calpastatin was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- Calpastatin Antigen Affinity purified polyclonal antibody
Calpastatin was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- Calpastatin Antigen Affinity purified polyclonal antibody
Calpastatin was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- Calpastatin Antigen Affinity purified polyclonal antibody
Calpastatin was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- Calpastatin Antigen Affinity purified polyclonal antibody

CAST / Calpastatin Antibody LS-C393264
Rabbit Polyclonal to Human CAST / Calpastatin
Mouse, Rat, Human
Flo IF IHC IHC-P WB
Unconjugated
100µl  $305.00

CAST / Calpastatin Antibody LS-C21149
Mouse Monoclonal (IgG1) to Human CAST / Calpastatin
Human
ELISA ICC IHC WB
Unconjugated
100µl  $620.00

CAST / Calpastatin Antibody LS-C660188
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Human
ELISA IF IHC WB
Unconjugated
100µg  $405.00

CAST / Calpastatin Antibody IHC-plus™ LS-B15589
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.

CAST / Calpastatin Antibody (clone CSL1-5) LS-C83579
Mouse Monoclonal [clone CSL1-5] (IgG1) to Human CAST / Calpastatin
Human
ELISA IHC IHC-Fr WB
Unconjugated
100µg  $455.00

CAST / Calpastatin Antibody (clone 2G11D6) LS-C84822
Mouse Monoclonal [clone 2G11D6] (IgG2a) to Bovine CAST / Calpastatin
Bovine
ICC IHC IHC-Fr WB
Unconjugated
100µl  $755.00

CAST / Calpastatin Antibody (clone 2C5) LS-C196815
Mouse Monoclonal [clone 2C5] (IgG1,k) to Human CAST / Calpastatin
Mouse, Human
ELISA IF WB
Unconjugated
100µg  $355.00
Immunofluorescence of monoclonal antibody to CAST on HeLa cell . [antibody concentration 10 ug/ml]
CAST monoclonal antibody (M03), clone 2C5. Western Blot analysis of CAST expression in NIH/3T3.
Immunofluorescence of monoclonal antibody to CAST on HeLa cell . [antibody concentration 10 ug/ml]
CAST monoclonal antibody (M03), clone 2C5. Western Blot analysis of CAST expression in NIH/3T3.

CAST / Calpastatin Antibody LS-C404761
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human cervical cancer using CAST Polyclonal Antibody at dilution of 1:60.
Immunohistochemistry of Human colon cancer using CAST Polyclonal Antibody at dilution of 1:60.
Immunohistochemistry of Human cervical cancer using CAST Polyclonal Antibody at dilution of 1:60.
Immunohistochemistry of Human colon cancer using CAST Polyclonal Antibody at dilution of 1:60.

CAST / Calpastatin Antibody LS-C401469
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human gastric cancer using CAST Polyclonal Antibody at dilution of 1:70.
Immunohistochemistry of Human colon cancer using CAST Polyclonal Antibody at dilution of 1:70.
Immunohistochemistry of Human gastric cancer using CAST Polyclonal Antibody at dilution of 1:70.
Immunohistochemistry of Human colon cancer using CAST Polyclonal Antibody at dilution of 1:70.

CAST / Calpastatin Antibody LS-C330790
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Mouse, Rat, Human
IHC WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded rat heart using CAST antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using CAST antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CAST antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using CAST antibodyat dilution of 1:100 (40x lens).

CAST / Calpastatin Antibody LS-C155216
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Human
IHC IHC-P WB
Unconjugated
100µl 0.7mg/ml  $335.00
IHC of paraffin-embedded Breast ca, using Calpastatin antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE CAST / Calpastatin antibody diluted at 1:2000
IHC of paraffin-embedded Breast ca, using Calpastatin antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE CAST / Calpastatin antibody diluted at 1:2000

CAST / Calpastatin Antibody LS-C748718
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Mouse, Rat, Human
IF WB
Unconjugated
50µl  $235.00
Immunofluorescence analysis of U2OS cells using CAST antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CAST antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Immunofluorescence analysis of U2OS cells using CAST antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CAST antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

CAST / Calpastatin Antibody (clone 1F7E3D10) LS-C84821
Mouse Monoclonal [clone 1F7E3D10] (IgG1) to Bovine CAST / Calpastatin
Bovine
ICC WB
Unconjugated
100µl  $695.00

CAST / Calpastatin Antibody LS-C306745
Mouse Polyclonal (IgG) to Human CAST / Calpastatin
Human
IF WB
Unconjugated
50µg  $560.00

CAST / Calpastatin Antibody LS-C668215
Rabbit Polyclonal to Human CAST / Calpastatin
Human
IP WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $275.00
Western blot Image
Western blot Image

CAST / Calpastatin Antibody LS-C309725
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Human
WB
Unconjugated
100µg  $605.00

CAST / Calpastatin Antibody (Biotin) LS-C521120
Rabbit Polyclonal (IgG) to Human CAST / Calpastatin
Human
WB
Biotin Conjugated
100µl  $755.00

CAST / Calpastatin Antibody LS-C668218
Rabbit Polyclonal to Human CAST / Calpastatin
Human
WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $275.00
Western blot Image
Western blot Image
Showing 1-25 of 30 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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