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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASP14 / Caspase 14 Antibody IHC-plus™

Note: This antibody replaces LS-C19463, LS-C56792, LS-C42489
Caspase 14 antibody LS-B474 is an unconjugated rabbit polyclonal antibody to Caspase 14 (CASP14) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B474-50
50 µg (1 mg/ml)
$425
Description
Caspase 14 antibody LS-B474 is an unconjugated rabbit polyclonal antibody to Caspase 14 (CASP14) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human CASP14 / Caspase 14
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A 16 amino acid synthetic peptide from near the carboxy terminus of human caspase-14. The immunogen is located within the last 50 amino acids of Caspase-14.
Blocking Peptide
LS-E29982 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B474. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
Caspase-14 antibody can be used for the detection of caspase-14 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 28 kDa Observed: 27 kDa
Presentation
PBS containing 0.02% thimerosal.
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About CASP14 / Caspase 14
P31944 NM_012114 NP_036246.1

Popular CASP14 / Caspase 14 Products

Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation
Western blot of Caspase-14. Tissue lysates (50 ug/lane) and recombinant human Caspase-14 were (Hu C14, 15 ng) were western blotted with Caspase-14 antibody at 1:2000. The antibody detected both the proform of caspase-14, and the large and small subunits of active/cleaved caspase-14.
Species: Human, Mouse, Rat, Dog, Gerbil
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Anti-Caspase 14 antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: ICC, Immunofluorescence, Flow Cytometry, ELISA

Publications (1)

1
A novel mechanism of filaggrin induction and sunburn prevention by beta -damascenone in Skh-1 mice. Uddin AN, Labuda I, Burns FJ. Toxicology and applied pharmacology. 2012 Dec;265:335-41. [Full Text Article] [PubMed:22982537]

Customer Reviews (0)


Images

Immunohistochemistry

Anti-Caspase 14 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 14 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 ug/ml.
Western blot of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 ug/ml.

Immunohistochemistry

Anti-Caspase 14 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 14 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 ug/ml.
Western blot of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 ug/ml.

Immunohistochemistry

Anti-Caspase 14 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 14 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 ug/ml.
Western blot of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 ug/ml.

Immunohistochemistry

Anti-Caspase 14 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 14 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 ug/ml.
Western blot of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 ug/ml.

Requested From: United States
Date Requested: 1/21/2019
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